Nanoscopy User Group

New group name! The STED User Group has become the Nanoscopy User Group

The focus of the Nanoscopy User Group is to create a collaborative space where researchers of all career stages can share their expertise using the latest super-resolution technologies. Sessions will be held on a variety of topics tailored to the needs of the audience, including best practices in experiments and data processing.

Next Meeting : 2026.11.12

 “Combining nanoscopy with spatial transcriptomics to understand gap junction networking of intercellular gene regulation in brain tissue” – Randy Stout, New York Institute of Technology 

Talk Abstract: I have been extending the utility of high-plex spatial transcriptomics by adding multi-channel immunostaining and other protein and lipid labeling tools with 3D super-resolution imaging (STED, SMLM, and array confocal). I will present our methodologies that I have developed in this area so far. The specific application for my lab’s research is to reveal the effects of gap junction networking of astrocytes in the brain on intercellular gene regulation.

Randy Stout is an Associate Professor at the New York Institute of Technology College of Osteopathic Medicine, Scientific Director of the Biomedical Research Innovation and Imaging Center (BRIIC), and the Director of the Imaging Center at New York Tech. My lab uses super-resolution microscopy to study nanoscale tissue morphology, gap junctions, astrocytes and thyroid hormone signaling in the brain. I use live-cell, high-resolution laser scanning confocal microscopy and super-resolution microscopy to reveal novel and fundamental aspects of the gap junction plaque structure (quasi-crystalline 2D protein arrays) and anatomy of other supramolecular cellular assemblies. I developed new optogenetic tools and methods for studying fluorescent protein tagged gap junction components during my graduate and postdoc research. Our recent work has provided evidence that gap junction endocytosis can facilitate intercellular mitochondria transfer that complemented parallel work using serial section electron microscopy in other labs.

Questions? bioimagingna.nanoscopyug@gmail.com

Register to join the group here!